Journal: Journal of hazardous materials
Article Title: A screening method for polyester films-degrading microorganisms and enzymes.
doi: 10.1016/j.jhazmat.2025.137177
Figure Lengend Snippet: Fig. 3. Phylogenetic tree of the selected esterases by 3D structures. The structure models of PCLD esterases were performed by using ColabFold. IsPETase (PDB:5YFE), LC-cutinase (PDB:4EB0) TfCut1(PDB:5ZOA) crystal structures were used for comparison. The phylogenetic analysis was conducted using the Dali server for comparing protein structures in 3D. The active esterases towards PET films are marked by blue, PLA – green and PHB/PHV – pink stars. The published PET- hydrolases are labelled by the red circle. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Previously published data demonstrated the hydrolytic activity of T. fusca cutinase (Addgene plasmid #76832) by detecting aromatic compounds after 48–72 hours of incubation with amorphous PET film and powder [25].
Techniques: Comparison